Action

Use holding pens or enclosures at release site prior to release of wild reptiles

How is the evidence assessed?
  • Effectiveness
    not assessed
  • Certainty
    not assessed
  • Harms
    not assessed

Study locations

Key messages

  • Seven studies evaluated the effects of using holding pens or enclosures at release sites prior to release of wild reptiles. Four studies were in the USA and one study was in each of Australia, New Zealand and the UK.

COMMUNITY RESPONSE (0 STUDIES)

POPULATION RESPONSE (5 STUDIES)

  • Reproductive success (1 study): One replicated, controlled study in New Zealand found that in a site where jewelled geckos were translocated into holding pens prior to release, more gravid females were found compared to a site where holding pens were not used.
  • Survival (4 studies): Two of three controlled studies (including one replicated study) in the USA and the UK found that gopher tortoises translocated into holding pens with artificial burrows prior to release or viviparous lizards released into an enclosure had higher survival (recaptured) or assumed survival (dug burrows) than individuals released without pens or enclosures. The other study found that translocating eastern box turtles into holding pens, or keeping them in pens for longer, did not affect post-release survival. One replicated study in the USA found that survival of Florida sand skinks within holding pens with different combinations of habitat features (trees, shade cloth, woody debris) ranged from 49–79% over two years.
  • Condition (1 study): One randomized, controlled study in the UK found that viviparous lizards released into an enclosure had similar body condition compared to those released without an enclosure.

BEHAVIOUR (4 STUDIES)

  • Behaviour change (4 studies): Two of three controlled studies (including two replicated studies) in the USA and New Zealand found that gopher tortoises and jewelled geckos translocated into holding pens prior to release dispersed away from the release site less frequently than those not held in pens. One study also found that the activity area of tortoises held in pens was smaller in the year of release, but similar in the year after release, compared to those not held in pens. The other study found that translocating eastern box turtles into holding pens, or keeping them in pens for longer, did not affect post-release dispersal behaviour. One controlled study in Australia found mixed effects on a range of behavioural measures of translocating pygmy bluetongue lizards into holding pens with artificial burrows for one day compared to five days.

About key messages

Key messages provide a descriptive index to studies we have found that test this intervention.

Studies are not directly comparable or of equal value. When making decisions based on this evidence, you should consider factors such as study size, study design, reported metrics and relevance of the study to your situation, rather than simply counting the number of studies that support a particular interpretation.

Supporting evidence from individual studies

  1. A replicated, controlled study in 1980–1982 in five areas of pine forest in Mississippi, USA (Lohoefener & Lohmeier 1986) found that translocations of gopher tortoises Gopherus polyphemus using release pens with artificial burrows prior to release were more successful than those that were not held initially in a release pen with burrows. Results were not statistically tested. When translocated gopher tortoises were initially held in release pens with artificial burrows, more tortoises were resighted or dug burrows during the 3–4-month monitoring period (17 of 21 recaptured or dug burrows, see paper for details) than when tortoises were released without a holding pen (directly released: 0 of 11; released in abandoned burrow: 1 of 5; released in artificial burrow: 0 of 3). Forty individually-marked adult gopher tortoises (some may have been captive releases) were translocated in spring–summer 1980–1982. Tortoises were released into artificial burrows in release pens (21 tortoises), directly released with no specific management (11 tortoises), into abandoned existing burrows (5 tortoises) or into artificial burrows (3 tortoises). Artificial burrows were 1 m deep and 45 degrees to the surface. Most tortoises (35 of 40) were released into areas with existing tortoise populations. Release pens were circular (4–7 m diameter), with translucent vinyl sheet walls (buried 10 cm into the ground) attached to wooden posts. Most tortoises were held in release pens for 2–4 weeks. Tortoises were monitored until late summer or early autumn in the release year.

    Study and other actions tested
  2. A replicated, controlled study in 1988–1993 in a recovering area of wetlands and mixed shrubland, grasses and trees in New York, USA (Cook 2004) found that releasing eastern box turtles Terrapene carolina carolina into holding pens prior to release did not affect post-release survival or dispersal. Annual survival was 71% and was not affected by spending time in a holding pen, neither was survival to two years (pen: 33%; no pen: 34%) or five years (pen: 27%; no pen: 24%). Post-release direction of dispersal (result presented as a bearing) and initial dispersal speed (days to disperse 100 m: average of 24–85 days) were also not affected by being in a holding pen prior to release. Nineteen gravid females, 11 clutches of 1–9 eggs, and 10 offspring were discovered following releases. In 1987–1990, a total of 335 turtles were collected from development sites or while crossing roads in suburban areas. Fifty-three turtles were fitted with radio trackers, and were either released immediately, or held in a pen for 15 days prior to release. The remaining 282 turtles were held in pens for 30 days before release. Originally a saltmarsh, the release site was created by dredge spoil deposition during 1928–1945. Radio tagged turtles were located daily for the first three days, then weekly until 1993. In 1993–1995, a trained dog Canis lupus familiaris was used to locate turtles.

    Study and other actions tested
  3. A controlled study in 2001–2003 in a mixed forest site in South Carolina, USA (Tuberville et al. 2005) found that releasing translocated gopher tortoises Gopherus polyphemus into a holding pen prior to release resulted in less dispersal away from the release site and smaller activity areas compared to when no holding pen was used.  More tortoises stayed at the released site when a release pen was used (9 months penning: 8 of 13, 62% stayed; 12 months penning: 11 of 12, 92% stayed) compared to when no pen was used (3 of 13, 23% stayed). In the year of release, tortoises held in pens for 12 months had smaller activity areas (2 ha) than those held for nine months (37 ha) or not held at all (94 ha), whereas in the year after release, activity areas were similar for all groups (5–40 ha). In 2001, tortoises were collected from an industrial development site. Groups of 12–13 adults and sub-adults were assigned either to a ‘soft release’ penning treatment (9 months or 12 months) or ‘hard release’ (no penning). All release areas contained 24 starter burrows. All turtles were released in 2002 and relocated in October–November 2002 and March–October 2003. Dispersers were retrieved and re-released at the release site.

    Study and other actions tested
  4. A controlled study in 2009 in a grassland enclosure in South Australia, Australia (Ebrahimi & Bull 2013) found that translocated pygmy bluetongue lizard Tiliqua adelaidensis confined to holding pens with artificial burrows for one day after release dispersed to marginal habitat less frequently and basked more than lizards confined for five days. After translocated pygmy bluetongue lizards were released from holding pens, lizards confined to a pen for one day dispersed to marginal habitat less frequently (0.2 lizards/cage/day) and basked for longer (22 minutes/hour) than lizards confined for five days (dispersal: 0.8 lizards/cage/day; basking time: 13 minutes/hour). Activity levels, movements, burrow switching, and agonistic interactions were similar between lizards confined for one or five days (see original paper for details). In October 2009, sixteen pygmy bluetongue lizards were captured in the wild and released into one of four predator-proof cages in a zoo enclosure (4 lizards/cage). Each cage included a central grassy circle (4 m diameter) with artificial burrows, surrounded by a strip of bare ground (5 m wide), encircled by a strip of marginal habitat (0.5 m wide) containing artificial burrows (see original paper for details of burrows). When lizards were released, all cages had a holding pen around the central grass areas. The pen was removed from two cages after one day and from the remaining two cages after five days. Lizard activity was monitored by video cameras over 10 days and analysis of lizard behaviour was based on observations from days 6–10 of the study.

    Study and other actions tested
  5. A replicated, controlled study in 2011–2012 in the Orokonui Valley, New Zealand (Knox & Monks 2014) found that keeping translocated jewelled geckos Naultinus gemmeus in a holding pen for up to ten months prior to release resulted in less movement away from their release site compared to unpenned geckos. None of the penned geckos (10 individuals) moved outside of the release area after the pen was removed (distance moved from release site: 1–16 m) compared to 67% (six of nine geckos) of the unpenned geckos (distance moved from release site: 4–39 m). Fourteen months after release, four females (all gravid) were found at the penned site and two (neither gravid) were found at the unpenned site. Forty-two geckos were translocated to Orokonui Ecosanctuary in December 2011 and January 2012 (21 females, six males and 15 unsexed juveniles) and held in a release pen (10–15 m wide, 55–60 m long and 0.5 m high) until September 2012, at which point the pen was removed. In September 2012, eleven individuals (six females, three males, two unsexed subadults) were released directly at a nearby site (200 m away). Ten penned and nine unpenned geckos were monitored by radio tracking (attached using a 22 x 3 cm self-adhesive fabric strip) one to two times daily for three weeks.

    Study and other actions tested
  6. A replicated study in 2008–2010 in dry scrubland in Florida, USA (McCoy et al. 2014) found that populations of Florida sand skinks Plestiodon reynoldsi translocated away from a proposed mining site and released into enclosures with different habitat features (trees, shade, woody debris) survived at least three years. Estimates of overall survival of translocated skinks ranged from 49–79%, and 105 of 300 skinks were recaptured during the two years following release. Provision of shade may have been important in explaining skink survival (reported as model result but effect size not reported). Newborn skinks (19 in 2008, 13 in 2009) were captured in all enclosure types. A further 35 newborns were trapped in 2010 (unpublished data). Skinks were sourced in spring 2007 from a site scheduled for sand mining and released in to fifteen 20 m2 enclosures (20 lizards/enclosure). Enclosures had five experimental treatments (tree only, shade cloth only, tree and coarse woody debris added, coarse woody debris only, control with no shade or debris). Skinks were trapped in enclosures in spring 2008–2009 (16 drift fences and 76 bucket-traps/enclosure), and further trapping was carried out in 2010 (method not given).

    Study and other actions tested
  7. A randomized, controlled study in 2016–2017 in an area of mixed grassland, scrub and woodland in Kent, UK (Nash 2017) found that more translocated viviparous lizards Zootoca vivipara were recaptured after release into an enclosure compared to those released in an unenclosed area, and that body condition was similar in the enclosed and unenclosed areas. More lizards were resighted after release in the enclosure (101 lizards) than in the unenclosed area (16 lizards). Body condition was similar for lizards in the enclosure and those in the unenclosed area (reported as condition index). Two adjacent sites in the wider release area (1.5 ha each) were selected, and one was randomly selected and enclosed with a reptile-proof fence (38 cm high, buried 30 cm deep). Both sites were provisioned with one hibernaculum and four earth banks. In 2016, lizards were translocated to both sites in the release area (total of 1,364 lizards) and 695 were released in the enclosure and 669 were released in the unenclosed area. In April–May 2017, translocated lizards were monitored at the two sites using visual encounter surveys and artificial cover boards (45/site).

    Study and other actions tested
Please cite as:

Sainsbury K.A., Morgan W.H., Watson M., Rotem G., Bouskila A., Smith R.K. & Sutherland W.J. (2021) Reptile Conservation: Global Evidence for the Effects of Interventions for reptiles. Conservation Evidence Series Synopsis. University of Cambridge, Cambridge, UK.

Where has this evidence come from?

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Reptile Conservation

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Reptile Conservation
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