Study

Conservation Cloning of vulnerable Esfahan mouflon (Ovis orientalis isphahanica): in vitro and in vivo studies

  • Published source details Hajian M., Hosseini S.M., Forouzanfar M., Abedi P., Ostadhosseini S., Hossieni L., Moulavi F., Gourabi H., Shahverdi A.H., Vosough Taghi Dizaj A., Kalantari S.A., Fotouhi Z., Iranpour R., Mahyar H., Amiri-Yekta A. & Nasr-Esfahani M.H. (2011) Conservation Cloning of vulnerable Esfahan mouflon (Ovis orientalis isphahanica): in vitro and in vivo studies. European Journal of Wildlife Research (formerly Zeitschrift für Jagdwissenschaft 1955-2003), 57, 959-969.

Actions

This study is summarised as evidence for the following.

Action Category

Clone rare species

Action Link
Terrestrial Mammal Conservation
  1. Clone rare species

    A controlled study (date not stated) in Iran (Hajian et al. 2011) found that immature eggs (oocytes) of domestic sheep have potential to be used for interspecies conservation cloning of Esfahan mouflon Ovis orientalis isphahanica. The success rate for transferring cell nuclei attached to Esfahan mouflon cells to domestic sheep oocytes (14.4%) did not significantly differ from that for transfer of nuclei attached to domestic sheep cells (22.1%). Subsequently, of 12 cloned mouflon blastocysts (early-stage cell mass which goes on to form an embryo) transferred to five domestic sheep recipients, two pregnancies resulted. In both cases live births of cloned Esfahan mouflon lambs resulted, but the lambs died soon after birth. Of 1,410 oocytes that had had their nucleus removed, 1,105 and 305 were attached to Esfahan mouflon and domestic sheep cells, respectively. Prior to transferring nuclei, donor cells were serum starved for 5 days. In vitro matured domestic sheep oocytes that had had their nucleus removed were then reconstituted with nuclei donor cells of mouflon and domestic sheep.

    (Summarised by: Nick Littlewood)

Output references
What Works 2021 cover

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence, in synopses. Subjects covered so far include amphibians, birds, mammals, forests, peatland and control of freshwater invasive species. More are in progress.

More about What Works in Conservation

Download free PDF or purchase
The Conservation Evidence Journal

The Conservation Evidence Journal

An online, free to publish in, open-access journal publishing results from research and projects that test the effectiveness of conservation actions.

Read the latest volume: Volume 18

Go to the CE Journal

Discover more on our blog

Our blog contains the latest news and updates from the Conservation Evidence team, the Conservation Evidence Journal, and our global partners in evidence-based conservation.


Who uses Conservation Evidence?

Meet some of the evidence champions

Endangered Landscape Programme Red List Champion - Arc Kent Wildlife Trust The Rufford Foundation Save the Frogs - Ghana Bern wood Supporting Conservation Leaders National Biodiversity Network Sustainability Dashboard Frog Life The international journey of Conservation - Oryx British trust for ornithology Cool Farm Alliance UNEP AWFA Butterfly Conservation People trust for endangered species Vincet Wildlife Trust