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Individual study: Hormonal induction of spermatozoa from amphibians with Rana temporaria and Bufo bufo as anuran models

Published source details

Uteshev V.K., Shishova N.V., Kaurova S.A., Browne R.K. & Gakhova E.N. (2012) Hormonal induction of spermatozoa from amphibians with Rana temporaria and Bufo bufo as anuran models. Reproduction, Fertility and Development, 24, 599–607


This study is summarised as evidence for the intervention(s) shown on the right. The icon shows which synopsis it is relevant to.

Use hormone treatment to induce sperm and egg release during captive breeding Amphibian Conservation

A replicated, controlled study in 2011 of captive amphibians in Russia (Uteshev et al. 2012) found that the greatest sperm production was induced with high dose lutenizing hormone-releasing hormone-a (LHRHa) for common frogs Rana temporaria and priming with LHRHa prior to human chorionic gonadotrophin (hCG) for common toads Bufo bufo. In common frogs, 1.2 μg/g bodyweight LHRHa induced significantly higher sperm numbers (650 x 106/ml) than pituitary extract (485 x 106) or 0.12 μg/g LHRHa (444 x 106), which produced significantly higher numbers than 23 IU/g hCG (170 x 106) and 12 IU/g hCG (39 x 106). High dose LHRHa had the highest percentage of samples with sperm concentrations above 200 x 106/ml (high LHRH: 40%; pituitaries: 36%; low LHRH: 15%; hCG: 0%). Sperm motility was similar with all treatments (76–90%). Priming common toads resulted in significantly higher numbers (11.6 x 106 vs 8.0 x 106/ml) and quality of sperm (motility: 85 vs 73%), but not higher sperm concentration (1.5 x 106 vs 1.8 x 106/ml). Four wild-caught frogs received each of the five hormone injection treatments. There were also 10 controls. Four wild-caught toads were primed with 0.13 μg/g LHRHa 24 hours before receiving 13 IU/g hCG; controls received only the second dose. Spermic urine was monitored.