Study

Hormonal induction of spermatozoa from amphibians with Rana temporaria and Bufo bufo as anuran models

  • Published source details Uteshev V.K., Shishova N.V., Kaurova S.A., Browne R.K. & Gakhova E.N. (2012) Hormonal induction of spermatozoa from amphibians with Rana temporaria and Bufo bufo as anuran models. Reproduction, Fertility and Development, 24, 599–607.

Actions

This study is summarised as evidence for the following.

Action Category

Amphibians: Use hormone treatment to induce sperm and egg release

Action Link
Management of Captive Animals

Use hormone treatment to induce sperm and egg release during captive breeding

Action Link
Amphibian Conservation
  1. Amphibians: Use hormone treatment to induce sperm and egg release

  2. Use hormone treatment to induce sperm and egg release during captive breeding

    A replicated, controlled study in 2011 of captive amphibians in Russia (Uteshev et al. 2012) found that the greatest sperm production was induced with high dose lutenizing hormone-releasing hormone-a (LHRHa) for common frogs Rana temporaria and priming with LHRHa prior to human chorionic gonadotrophin (hCG) for common toads Bufo bufo. In common frogs, 1.2 μg/g bodyweight LHRHa induced significantly higher sperm numbers (650 x 106/ml) than pituitary extract (485 x 106) or 0.12 μg/g LHRHa (444 x 106), which produced significantly higher numbers than 23 IU/g hCG (170 x 106) and 12 IU/g hCG (39 x 106). High dose LHRHa had the highest percentage of samples with sperm concentrations above 200 x 106/ml (high LHRH: 40%; pituitaries: 36%; low LHRH: 15%; hCG: 0%). Sperm motility was similar with all treatments (76–90%). Priming common toads resulted in significantly higher numbers (11.6 x 106 vs 8.0 x 106/ml) and quality of sperm (motility: 85 vs 73%), but not higher sperm concentration (1.5 x 106 vs 1.8 x 106/ml). Four wild-caught frogs received each of the five hormone injection treatments. There were also 10 controls. Four wild-caught toads were primed with 0.13 μg/g LHRHa 24 hours before receiving 13 IU/g hCG; controls received only the second dose. Spermic urine was monitored.

     

Output references
What Works 2021 cover

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence, in synopses. Subjects covered so far include amphibians, birds, mammals, forests, peatland and control of freshwater invasive species. More are in progress.

More about What Works in Conservation

Download free PDF or purchase
The Conservation Evidence Journal

The Conservation Evidence Journal

An online, free to publish in, open-access journal publishing results from research and projects that test the effectiveness of conservation actions.

Read the latest volume: Volume 18

Go to the CE Journal

Discover more on our blog

Our blog contains the latest news and updates from the Conservation Evidence team, the Conservation Evidence Journal, and our global partners in evidence-based conservation.


Who uses Conservation Evidence?

Meet some of the evidence champions

Endangered Landscape Programme Red List Champion - Arc Kent Wildlife Trust The Rufford Foundation Save the Frogs - Ghana Bern wood Supporting Conservation Leaders National Biodiversity Network Sustainability Dashboard Frog Life The international journey of Conservation - Oryx British trust for ornithology Cool Farm Alliance UNEP AWFA Butterfly Conservation People trust for endangered species Vincet Wildlife Trust