Study

Immunization with formalin-killed chytrid fungus had no effect on time to infection, infection prevalence or intensity or mortality in mountain yellow-legged frogs

  • Published source details Stice M.J. & Briggs C.J. (2010) Immunization is ineffective at preventing infection and mortality due to the amphibian chytrid fungus Batrachochytrium dendrobatidis. Journal of Wildlife Diseases, 46, 70-77

Actions

This study is summarised as evidence for the following.

Action Category

Immunize amphibians against chytridiomycosis infection

Action Link
Amphibian Conservation
  1. Immunize amphibians against chytridiomycosis infection

    A randomized, replicated, controlled study in a laboratory at the University of California, USA (Stice & Briggs 2010) found that vaccinating mountain yellow-legged frogs Rana muscosa with formalin-killed chytrid fungus did not significantly reduce infection rate with chytridiomycosis or mortality. The proportion of frogs that became infected (chytrid/adjuvant: 0.8; adjuvant only: 0.9; control: 0.8) and died (chytrid/adjuvant: 0.4; adjuvant: 0.4; control: 0.2) were similar to controls. Following vaccination, there was no significant difference in the time to infection, rate of increase in chytrid zoospores in animals (chytrid/adjuvant: 0.08; adjuvant: 0.08; control: 0.09) or the maximum number of zoospores per frog (chytrid/adjuvant: 53,990; adjuvant: 17,831; control: 5,106). Frogs were randomly assigned into three groups of 19–20 individuals. Controls received an injection of saline. One group received a 1:1 vaccination of formalin-killed chytrid fungus in Freund’s complete adjuvant (to increase effectiveness) and one month later formalin-killed chytrid in Freund’s incomplete adjuvant. Another group received saline with Freund’s complete adjuvant and one month later saline with Freund’s incomplete adjuvant. Injections comprised 0.05 cm³ into the dorsal lymph sac. Frogs were exposed to live chytrid (105 zoospores) one month after treatments. Individuals were monitored weekly for chytridiomycosis using swabs of the ventral surface.

     

Output references

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