Study

Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther’s toadlet, Pseudophryne guentheri

  • Published source details Silla A.J. (2010) Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther’s toadlet, Pseudophryne guentheri. Reproductive Biology and Endocrinology, 8, 139-147

Actions

This study is summarised as evidence for the following.

Action Category

Amphibians: Use hormone treatment to induce sperm and egg release

Action Link
Management of Captive Animals

Use hormone treatment to induce sperm and egg release during captive breeding

Action Link
Amphibian Conservation
  1. Amphibians: Use hormone treatment to induce sperm and egg release

  2. Use hormone treatment to induce sperm and egg release during captive breeding

    A replicated, controlled study in 2009 of captive Gϋnther’s toadlets Pseudophryne guentheri in Western Australia (Silla 2010) found that hormone treatment successfully induced sperm release. Luteinizing hormone-releasing hormone (LHRHa) in doses of 1, 2, 4 or 8 μg/g induced 100% of males to produce sperm, compared to 10–30% of controls. Numbers of sperm released was significantly higher following 2 μg/g of LHRHa (25 x 103) than 8 μg/g (5 x 103) or controls (0); other doses did not differ significantly (8–12 x 103). Sperm viability was significantly higher following the 1 μg/g compared to 8 μg/g treatment. Arg8-vasotocin acetate salt (4 μg/g) alone or with 2 μg/g LHRHa resulted in similar numbers of males releasing sperm as a single 2 μg/g dose of LHRHa (71; 71; 100% respectively). However, sperm numbers were significantly lower (0 vs 25 x 103). Male toadlets were given a single dose of 1, 2, 4 or 8 μg/g bodyweight of LHRHa in simplified amphibian Ringer solution, or a control of 100 μL of simplified amphibian Ringer solution (n = 7–10/treatment). Sperm release was tested at 3, 7 and 12 hours post-treatment.

     

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