Individual study: Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther’s toadlet, Pseudophryne guentheri
Silla A.J. (2010) Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther’s toadlet, Pseudophryne guentheri. Reproductive Biology and Endocrinology, 8, 139-147
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Use hormone treatment to induce sperm and egg release during captive breeding
A replicated, controlled study in 2009 of captive Gϋnther’s toadlets Pseudophryne guentheri in Western Australia (Silla 2010) found that hormone treatment successfully induced sperm release. Luteinizing hormone-releasing hormone (LHRHa) in doses of 1, 2, 4 or 8 μg/g induced 100% of males to produce sperm, compared to 10–30% of controls. Numbers of sperm released was significantly higher following 2 μg/g of LHRHa (25 x 103) than 8 μg/g (5 x 103) or controls (0); other doses did not differ significantly (8–12 x 103). Sperm viability was significantly higher following the 1 μg/g compared to 8 μg/g treatment. Arg8-vasotocin acetate salt (4 μg/g) alone or with 2 μg/g LHRHa resulted in similar numbers of males releasing sperm as a single 2 μg/g dose of LHRHa (71; 71; 100% respectively). However, sperm numbers were significantly lower (0 vs 25 x 103). Male toadlets were given a single dose of 1, 2, 4 or 8 μg/g bodyweight of LHRHa in simplified amphibian Ringer solution, or a control of 100 μL of simplified amphibian Ringer solution (n = 7–10/treatment). Sperm release was tested at 3, 7 and 12 hours post-treatment.