Study

Cryopreservation of spermatozoa of the terrestrial Puerto Rican frog, Eleutherodactylus coqui

  • Published source details Michael S.F. & Jones C. (2004) Cryopreservation of spermatozoa of the terrestrial Puerto Rican frog, Eleutherodactylus coqui. Cryobiology, 48, 90-94

Actions

This study is summarised as evidence for the following.

Action Category

Amphibians: Freeze sperm or eggs for future use

Action Link
Management of Captive Animals

Freeze sperm or eggs for future use

Action Link
Amphibian Conservation
  1. Amphibians: Freeze sperm or eggs for future use

  2. Freeze sperm or eggs for future use

    A replicated study of captive Puerto Rican frogs Eleutherodactylus coqui in the USA (Michael & Jones 2004) found that cryopreservation of sperm was successful with a cryoprotectant and fetal bovine serum (FSB). FBS alone resulted in only 8% viability. However, sperm viability was significantly higher with addition of sucrose or glycerol to FBS (sucrose: 28%; glycerol: 30%; dimethyl sulfoxide: 20%). Viability did not differ significantly with dimethyl sulfoxide. Prior to freezing sperm had a viability of 56% and so normalized viabilities were: 14% for FBS alone and 35%, 50% and 54% with added dimethyl sulfoxide, sucrose and glycerol respectively. Testes of wild caught frogs were macerated in solution. Sperm was then mixed with a cryoprotectant solution (six replicates/treatment): heat inactivated FBS alone, FBS with 2M sucrose, FBS with 2M glycerol or FBS with 2M dimethyl sulfoxide. Mixtures were frozen at −80°C for 24 hours and then thawed rapidly in a 20°C water bath. Fluorescent dye was used to examine sperm.

     

Output references

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence, in synopses. Subjects covered so far include amphibians, birds, forests, peatland and control of freshwater invasive species. More are in progress.

More about What Works in Conservation

Download free PDF or purchase
The Conservation Evidence Journal

The Conservation Evidence Journal

An online, free to publish in, open-access journal publishing results from research and projects that test the effectiveness of conservation actions.

Read the latest volume: Volume 18

Go to the CE Journal

Discover more on our blog

Our blog contains the latest news and updates from the Conservation Evidence team, the Conservation Evidence Journal, and our global partners in evidence-based conservation.


Who uses Conservation Evidence?

Meet some of the evidence champions

Endangered Landscape Programme Red List Champion - Arc Kent Wildlife Trust The Rufford Foundation Save the Frogs - Ghana Bern wood Supporting Conservation Leaders National Biodiversity Network Sustainability Dashboard Frog Life The international journey of Conservation - Oryx British trust for ornithology Cool Farm Alliance UNEP AWFA Butterfly Conservation People trust for endangered species Vincet Wildlife Trust