Study

Motility and cryopreservation of spermatozoa of European common frog, Rana temporaria

  • Published source details Mansour N., Lahnsteiner F. & Patzner R.A. (2010) Motility and cryopreservation of spermatozoa of European common frog, Rana temporaria. Theriogenology, 74, 724-732

Actions

This study is summarised as evidence for the following.

Action Category

Freeze sperm or eggs for future use

Action Link
Amphibian Conservation

Use hormone treatment to induce sperm and egg release during captive breeding

Action Link
Amphibian Conservation
  1. Freeze sperm or eggs for future use

    A replicated study in 2009 of captive European common frogs Rana temporaria in Austria (Mansour, Lahnsteiner & Patzner 2010) found that the most effective cryopreservation protocol was sperm in motility-inhibiting saline (MIS) with 5% glycerol, 2.5% sucrose and 5% hen egg yolk, frozen 10 cm above liquid nitrogen and thawed at 22 °C for 40 seconds. Sperm motility was maintained following incubation for 40 minutes at 4°C in MIS with 10% dimethyl sulfoxide (71%), 5% glycerol (69%) or 10% methanol (59%), but not 10% propandiol (0%). When frozen, in combination with sucrose, dimethyl sulfoxide resulted in significantly greater sperm motility and viability (10%; 42% respectively) than glycerol (8%; 25%). With MIS, motility and viability was similar with either dimethyl sulfoxide (13%; 27%) or glycerol (10%; 29%). Sperm frozen in MIS with sucrose and methanol had no motility. Sperm frozen 5 cm above liquid nitrogen had no motility, whereas at 10 cm motility was 30–35%. Addition of 5% (vs 10%) egg yolk and 2.5% sucrose to MIS with glycerol significantly increased hatching rate compared to all other treatments (23 vs 2–12%). Motility and viability did not differ. Testes from wild males were macerated (3/treatment). Sperm was frozen in liquid nitrogen. Fertilization was tested using 25–30 eggs.

     

  2. Use hormone treatment to induce sperm and egg release during captive breeding

    A replicated study in 2009 of captive European common frogs Rana temporaria in Austria (Mansour, Lahnsteiner & Patzner 2010) found that injecting males with human chorionic gonadotrophin increased sperm production. Males stimulated with hormones had greater sperm production than untreated males (0.004 vs 0.002 testis/body weight). The same was true for the sperm cell concentration (80 vs 11 x 106/ml in 1.5 ml motility-inhibiting saline/testes). Males received injections of 150 IU of human chorionic gonadotrophin and were killed after 15 hours. Testes were removed weighed and macerated in motility-inhibiting saline.

     

Output references

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