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Providing evidence to improve practice

Individual study: Unlikely remedy: fungicide clears infection from pathogenic fungus in larval southern leopard frogs (Lithobates sphenocephalus)

Published source details

Hanlon S.M., Kerby J.L. & Parris M.J. (2012) Unlikely remedy: fungicide clears infection from pathogenic fungus in larval southern leopard frogs (Lithobates sphenocephalus). PLoS ONE, 7, e43573


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Use antifungal treatment to reduce chytridiomycosis infection Amphibian Conservation

A randomized, replicated, controlled study in 2010 of captive amphibians in Tennessee, USA (Hanlon, Kerby & Parris 2012) found that southern leopard frog tadpoles Lithobates sphenocephalus treated with thiophanate-methyl (TM) were cured of chytridiomycosis. All treated tadpoles tested negative for the infection at day 60, as did controls. All infected untreated tadpoles tested positive. By day 60, treated tadpoles were significantly heavier (TM + chytrid: 2.0; TM: 1.1; controls: 0.8–0.9 g) and longer (TM + chytrid: 22; TM: 18; controls: 17 mm). The same was true for metamorphosis mass (TM + chytrid: 1.1; TM: 0.9; controls: 0.5–0.7 g) and length (TM + chytrid: 23; TM: 22; controls: 18–19 mm). Ten tadpoles were randomly assigned to each treatment: thiophanate-methyl treatment of chytrid infected tadpoles, thiophanate-methyl treatment alone, chytrid infection alone and an uninfected control group. Tadpoles were bathed in thiophanate-methyl (0.6 mg/L) and water was changed every three days. Animals were measured and tested for infection at day 60 and measured on tail resorption.