Guidelines for the safe use of disposable gloves with amphibian larvae in light of pathogens and possible toxic effects

  • Published source details Greer A.L., Schock D.M., Brunner J.L., Johnson R.A., Picco A.M., Cashins S.D., Alford R.A., Skerratt L.F. & Collins J.P. (2009) Guidelines for the safe use of disposable gloves with amphibian larvae in light of pathogens and possible toxic effects. Herpetological Review, 40, 145-147.


This study is summarised as evidence for the following.

Action Category

Use gloves to handle amphibians

Action Link
Amphibian Conservation
  1. Use gloves to handle amphibians

    A review of 22 amphibian species in laboratory experiments, in the field and in zoo settings in Canada and the USA (Greer et al. 2009) found that there were no adverse effects of handling amphibians using disposable gloves. No effects were noticed in wood frogs Rana sylvatica (n = 240), Arizona tiger salamanders Ambystoma tigrinum nebulosum (n = 1372) or gray tiger salamanders Ambystoma tigrinum diaboli (n = 397) handled for up to three minutes, weekly for 4–20 weeks in laboratories. The same was true for wood frogs (n = 32), western toads Bufo boreas (n = 98), boreal choral frogs Pseudacris maculata (n = 4) and Arizona tiger salamanders Ambytoma tirgrinum nebulosum (n = 2309) handled for up to two minutes in the field. In addition, no symptoms or deaths were ever detected in the larvae of 17 amphibian species that had been repeatedly handled with gloves at Detroit Zoo.

Output references
What Works 2021 cover

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence, in synopses. Subjects covered so far include amphibians, birds, mammals, forests, peatland and control of freshwater invasive species. More are in progress.

More about What Works in Conservation

Download free PDF or purchase
The Conservation Evidence Journal

The Conservation Evidence Journal

An online, free to publish in, open-access journal publishing results from research and projects that test the effectiveness of conservation actions.

Read the latest volume: Volume 18

Go to the CE Journal

Discover more on our blog

Our blog contains the latest news and updates from the Conservation Evidence team, the Conservation Evidence Journal, and our global partners in evidence-based conservation.

Who uses Conservation Evidence?

Meet some of the evidence champions

Endangered Landscape Programme Red List Champion - Arc Kent Wildlife Trust The Rufford Foundation Save the Frogs - Ghana Bern wood Supporting Conservation Leaders National Biodiversity Network Sustainability Dashboard Frog Life The international journey of Conservation - Oryx British trust for ornithology Cool Farm Alliance UNEP AWFA Butterfly Conservation People trust for endangered species Vincet Wildlife Trust