Individual study: Using itraconazole to clear Batrachochytrium dendrobatidis infection, and subsequent depigmentation of Alytes muletensis tadpoles
Garner T., Garcia G., Carroll B. & Fisher M. (2009) Using itraconazole to clear Batrachochytrium dendrobatidis infection, and subsequent depigmentation of Alytes muletensis tadpoles. Diseases of Aquatic Organisms, 83, 257–260
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Use antifungal treatment to reduce chytridiomycosis infection
A randomized, replicated, controlled study in England, UK (Garner et al. 2009) found that treatment with itraconazole cured all captive Mallorcan midwife toad Alytes muletensis tadpoles of chytridiomycosis, but caused depigmentation. All treated tadpoles tested negative for chytrid infection. However, tadpoles showed significant depigmentation in all treatments and some controls. Fifteen of 17 infected control tadpoles tested positive for infection over 21 days. Tadpoles were infected over two weeks then randomly assigned to treatments. Nine treatment groups of six tadpoles were treated with itraconazole baths of 0.5, 1.0 or 1.5 mg/L over 7, 14 or 21 days. Tadpoles were killed humanely one week later. Three control groups of 4–5 infected tadpoles were euthanized at 14, 21 or 28 days post-treatment to test for infection.