Individual study: Successful recovery of motility and fertility of cryopreserved cane toad (Bufo marinus) sperm
Browne R.K., Clulow J., Mahony M. & Clark A. (1998) Successful recovery of motility and fertility of cryopreserved cane toad (Bufo marinus) sperm. Cryobiology, 37, 339-345
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Freeze sperm or eggs for future use
A replicated study of cane toads Bufo marinus in Australia (Browne et al. 1998) found that sperm retained motility and fertilizing capacity following cryopreservation, provided that cryoprotectants were used. Sperm frozen in sucrose alone retained no motility. The highest rates of recovery of sperm motility and fertilizing capacity were observed following storage with 15% dimethyl sulfoxide (motility: 69%; fertilization: 61%) and 20% glycerol (motility: 58%; fertilization: 81%). However, storage with different concentrations of dimethyl sulfoxide or glycerol all showed some motility (dimethyl sulfoxide: 35–69%; glycerol: 15–58%) and fertilizing capacity (dimethyl sulfoxide: 33-61%; glycerol: 15–81%). Sperm from macerated testes of four toads were cryopreserved in suspensions of 10% sucrose alone or with 10, 15 or 20% dimethyl sulfoxide or glycerol. Suspensions were cooled slowly to −196 °C. Sperm was thawed in air and tested within five minutes for motility and fertilization capacity (eggs from two females).