Short-term storage of cane toad (Bufo marinus) gametes
Published source details
Browne R.K., Clulow J. & Mahony M. (2001) Short-term storage of cane toad (Bufo marinus) gametes. Reproduction, 121, 167-173
Published source details Browne R.K., Clulow J. & Mahony M. (2001) Short-term storage of cane toad (Bufo marinus) gametes. Reproduction, 121, 167-173
This study is summarised as evidence for the following.
Amphibians: Freeze sperm or eggs for future useAction Link
Freeze sperm or eggs for future useAction Link
Amphibians: Freeze sperm or eggs for future use
Freeze sperm or eggs for future use
A replicated study of captive cane toads Bufo marinus in Australia (Browne, Clulow & Mahony 2001) found that storage method and temperature affected sperm and egg viability. Sperm stored in testes showed greater than 50% motility for seven days at 0°C and five days at 4°C. By day 15 only sperm stored at 0°C showed any motility (3%). In suspension, the longest retention of motility and fertilizing capacity was following storage in concentrated (1:1 dilution) anaerobic suspensions (up to 25–30 days). However, fertilization rates were significantly higher following storage in 1:5 dilution (day 5: 85% vs 55% for other concentrations). Egg viability was significantly higher following storage at 15°C compared to other temperatures (8 hours: 90% vs 0–60%). Storage at 5°C resulted in a decline to 0% viability after two hours. Sperm from wild toads were stored in intact testes at 0 or 4°C for 15 days (n = 6/treatment) or in suspension (macerated testes; n = 24) with Simplified amphibian Ringer solution at 0°C for 30 days. Dilutions were 1:1, 1:5 or 1:10 (testes:solution) and storage tubes were either opened or sealed. Immediately after ovulation, eggs from three females were stored in simplified amphibian Ringer solution at 5, 10, 15, 20 and 25°C (1,500 eggs/female). Fertilization rate was monitored up until 12 hours.