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Providing evidence to improve practice

Individual study: Dimethyl sulfoxide successfully preserves American kestrel Falco sparverius semen for artificial insemination

Published source details

Gee G.F., Morrell C.A., Franson J.C. & Pattee O.H. (1993) Cryopreservation of American kestrel semen with dimethylsulfoxide. Journal of Raptor Research, 27, 21-25


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Freeze semen for use in artificial insemination Bird Conservation

A small ex situ study in 1983-5 (Gee et al. 1993) found that six American kestrels Falco sparverius inseminated with previously-frozen semen produced 14 infertile eggs only when the semen contained 4% dimethyl sulphoxide (DMSO). This compared with 35% of 17 eggs being fertile when 6% DMSO was used; 40% of ten eggs for 8% DMSO and 57% of seven eggs for 10% DMSO. Sperm mobility in semen containing 10% DMSO was lower (44%) than in semen containing 8% or 6% DMSO (61% and 62% respectively). Semen was taken from 15 male kestrels and frozen for between one and 14 months prior to thawing and insemination.