Rapid in vitro propagation of Premna serratifolia, a medicinally important declining shrub, India

  • Published source details Chinnappan R.S., Ruthar N. & Sethu S.S. (2011) Rapid in vitro propagation of Premna serratifolia, a medicinally important declining shrub, India. Conservation Evidence, 8, 66-73.


A protocol for micropropagation of the medicinally important plant Premna serratifolia was developed due to its increasing rarity as a result of over-exploitation and poor natural regeneration within its range of occurrence in the Indian sub-continent. Plantlets were regenerated through shoot tip explants. Shoot tip explants were cultured on different media (MS, SH, Y3 and B5). Anti-oxidants (activated charcoal, citric acid, polyvinylpyrrolidone) and the effect of seasonal changes (through out the year) were analyzed for reducing explant browning and better shoot multiplication. The highest number of shoots was developed from MS medium supplemented with BAP (3.0 mg/l), IAA (0.5 mg/l) and activated charcoal (10.0 mg/l) between November and March. Best rooting was achieved from the medium supplemented with NAA (1.0 mg/l). Complete regeneration was achieved in about 21 days. The plantlets, thus developed were maintained under controlled conditions in the green house for 40 days. They were then planted out into a nursery where growth has been good and survival, up to 1 year, has been high (95%).

Output references
What Works 2021 cover

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence, in synopses. Subjects covered so far include amphibians, birds, mammals, forests, peatland and control of freshwater invasive species. More are in progress.

More about What Works in Conservation

Download free PDF or purchase
The Conservation Evidence Journal

The Conservation Evidence Journal

An online, free to publish in, open-access journal publishing results from research and projects that test the effectiveness of conservation actions.

Read the latest volume: Volume 20

Go to the CE Journal

Discover more on our blog

Our blog contains the latest news and updates from the Conservation Evidence team, the Conservation Evidence Journal, and our global partners in evidence-based conservation.

Who uses Conservation Evidence?

Meet some of the evidence champions

Endangered Landscape Programme Red List Champion - Arc Kent Wildlife Trust The Rufford Foundation Save the Frogs - Ghana Bern wood Supporting Conservation Leaders National Biodiversity Network Sustainability Dashboard Frog Life The international journey of Conservation - Oryx British trust for ornithology Cool Farm Alliance UNEP AWFA Bat Conservation InternationalPeople trust for endangered speciesVincet Wildlife Trust