Individual study: Rapid in vitro propagation of Premna serratifolia, a medicinally important declining shrub, India
Chinnappan R.S., Ruthar N. & Sethu S.S. (2011) Rapid in vitro propagation of Premna serratifolia, a medicinally important declining shrub, India. Conservation Evidence, 8, 66-73
A protocol for micropropagation of the medicinally important plant Premna serratifolia was developed due to its increasing rarity as a result of over-exploitation and poor natural regeneration within its range of occurrence in the Indian sub-continent. Plantlets were regenerated through shoot tip explants. Shoot tip explants were cultured on different media (MS, SH, Y3 and B5). Anti-oxidants (activated charcoal, citric acid, polyvinylpyrrolidone) and the effect of seasonal changes (through out the year) were analyzed for reducing explant browning and better shoot multiplication. The highest number of shoots was developed from MS medium supplemented with BAP (3.0 mg/l), IAA (0.5 mg/l) and activated charcoal (10.0 mg/l) between November and March. Best rooting was achieved from the medium supplemented with NAA (1.0 mg/l). Complete regeneration was achieved in about 21 days. The plantlets, thus developed were maintained under controlled conditions in the green house for 40 days. They were then planted out into a nursery where growth has been good and survival, up to 1 year, has been high (95%).