Individual study: Treatment with carbon dioxide induces egg-laying in captive buff-tailed bumblebee queens Bombus terrestris; a laboratory study in Würzburg, Bayern, Germany
Röseler P.F. (1985) A technique for year-round rearing of Bombus terrestris (Apidae, Bombini) colonies in captivity. Apidologie, 16, 165-170
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Rear declining bumblebees in captivity
In a replicated trial in Germany, Röseler (1985) demonstrated that mated queen B. terrestris can be induced to lay eggs by anaesthestising them with CO2 for 30 minutes on two consecutive days. After this treatment, 73% of unhibernated and 81% of hibernated queens began egg-laying within one week of confinement with workers.