Collected Evidence: Collected Evidence: Add salt to ponds to reduce chytridiomycosis One study in Australia found that following addition of salt to a pond containing the chytrid fungus, a population of green and golden bell frogs remained free of chytridiomycosis for at least six months.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F762https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F762Fri, 16 Aug 2013 14:13:19 +0100Collected Evidence: Collected Evidence: Use antibacterial treatment to reduce chytridiomycosis infection Two studies (including one randomized, replicated, controlled study) in New Zealand and Australia found that treatment with chloramphenicol antibiotic ointment (Bishop et al. 2009) or solution, with other interventions in some cases, cured green tree frogs and one Archey’s frog of chytridiomycosis. One replicated, controlled study found that treatment with trimethoprim-sulfadiazine increased survival time but did not cure blue-and-yellow poison dart frogs of chytridiomycosis.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F763https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F763Fri, 16 Aug 2013 14:30:56 +0100Collected Evidence: Collected Evidence: Use antifungal skin bacteria or peptides to reduce chytridiomycosis infection Three of four randomized, replicated, controlled studies in the USA found that adding antifungal bacteria to the skin of salamanders or frogs exposed to the chytrid fungus did not reduce chytridiomycosis infection rate or death. One found that adding antifungal bacteria to frogs prevented infection and death. One randomized, replicated, controlled study in the USA found that adding antifungal skin bacteria to soil significantly reduced chytridiomycosis infection rate of red-backed salamanders. One randomized, replicated, controlled study in Switzerland found that treatment with antimicrobial skin peptides before or after infection with chytridiomycosis did not significantly increase survival of common toads. Three randomized, replicated, controlled studies in the USA found that adding antifungal skin bacteria to chytrid infected amphibians reduced weight loss.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F764https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F764Fri, 16 Aug 2013 15:10:05 +0100Collected Evidence: Collected Evidence: Immunize amphibians against chytridiomycosis infection One randomized, replicated, controlled study in the USA found that vaccinating mountain yellow-legged frogs with formalin-killed chytrid fungus did not significantly reduce chytridiomycosis infection rate or mortality.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F765https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F765Fri, 16 Aug 2013 16:05:06 +0100Collected Evidence: Collected Evidence: Remove the chytrid fungus from ponds One before-and-after study in Mallorca found that pond drying and fungicidal treatment of resident midwife toads reduced levels of infection but did not eradicate chytridiomycosis.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F766https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F766Fri, 16 Aug 2013 16:11:48 +0100Collected Evidence: Collected Evidence: Treat amphibians with chytridiomycosis in the wild or pre-release One before-and-after study in Mallorca found that treating wild midwife toads with fungicide, along with pond drying, reduced infection levels but did not eradicate chytridiomycosis.    Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F767https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F767Fri, 16 Aug 2013 16:18:57 +0100Collected Evidence: Collected Evidence: Sterilize equipment when moving between amphibian sites We found no evidence for the effects of sterilizing equipment when moving between amphibian sites on the spread of disease between amphibian populations or individuals. Two randomized, replicated, controlled study in Switzerland and Sweden found that Virkon S disinfectant did not affect survival, mass or behaviour of common frog or common toad tadpoles or moor frog embryos or hatchlings. One of the studies found that bleach significantly reduced survival of common frog and common toad tadpoles.    Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F768https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F768Fri, 16 Aug 2013 16:21:14 +0100Collected Evidence: Collected Evidence: Use gloves to handle amphibians We found no evidence for the effects of using gloves on the spread of disease between amphibian populations or individuals. A review for Canada and the USA found that there were no adverse effects of handling 22 amphibian species using disposable gloves. However, three replicated studies (including one controlled study) in Australia and Austria found that deaths of tadpoles were caused by latex gloves for all four species tested, by vinyl gloves for three of five species and by nitrile gloves for the one species tested.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F769https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F769Mon, 19 Aug 2013 15:45:10 +0100Collected Evidence: Collected Evidence: Use temperature treatment to reduce chytridiomycosis infection Four of five studies (including four replicated, controlled studies) in Australia, Switzerland and the USA found that increasing enclosure or water temperature to 30–37°C for over 16 hours cured frogs and toads of chytridiomycosis. One found that heat treatment at 30–35°C for 36 hours did not cure northern leopard frogs.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F770https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F770Mon, 19 Aug 2013 16:15:16 +0100Collected Evidence: Collected Evidence: Use zooplankton to remove zoospores We found no evidence for the effects of using zooplankton to remove chytrid zoospores on amphibian populations. 'No evidence' for an action means we have not yet found any studies that directly and quantitatively tested this action during our systematic journal and report searches. Therefore we have been unable to assess whether or not the action is effective or has any harmful impacts. Please get in touch if you know of such a study for this action.    Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F800https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F800Thu, 22 Aug 2013 14:44:57 +0100Collected Evidence: Collected Evidence: Use antifungal treatment to reduce chytridiomycosis infection Twelve of 16 studies (including four randomized, replicated, controlled studies) in Europe, Australia, Tasmania, Japan and the USA found that antifungal treatment cured or increased survival of amphibians with chytridiomycosis. Four studies found that treatments did not cure chytridiomycosis, but did reduce infection levels or had mixed results. Six of the eight studies (including two randomized, replicated, controlled studies) in Japan, Tasmania, the UK and USA testing treatment with itraconazole found that it was effective at curing amphibians of chytridiomycosis. One study found that it reduced infection levels and one found mixed effects. Six studies found that specific fungicides caused death or other negative side effects in amphibians.  Collected Evidencehttps%3A%2F%2Fwww.conservationevidence.com%2Factions%2F882https%3A%2F%2Fwww.conservationevidence.com%2Factions%2F882Fri, 13 Sep 2013 13:44:14 +0100
What Works 2021 cover

What Works in Conservation

What Works in Conservation provides expert assessments of the effectiveness of actions, based on summarised evidence, in synopses. Subjects covered so far include amphibians, birds, mammals, forests, peatland and control of freshwater invasive species. More are in progress.

More about What Works in Conservation

Download free PDF or purchase
The Conservation Evidence Journal

The Conservation Evidence Journal

An online, free to publish in, open-access journal publishing results from research and projects that test the effectiveness of conservation actions.

Read the latest volume: Volume 21

Go to the CE Journal

Discover more on our blog

Our blog contains the latest news and updates from the Conservation Evidence team, the Conservation Evidence Journal, and our global partners in evidence-based conservation.


Who uses Conservation Evidence?

Meet some of the evidence champions

Endangered Landscape ProgrammeRed List Champion - Arc Kent Wildlife Trust The Rufford Foundation Save the Frogs - Ghana Mauritian Wildlife Supporting Conservation Leaders
Sustainability Dashboard National Biodiversity Network Frog Life The international journey of Conservation - Oryx Cool Farm Alliance UNEP AWFA Bat Conservation InternationalPeople trust for endangered species Vincet Wildlife Trust